Optimization of space maneuvers is becoming a key requirement for the evolution of the space market and paves the way towards new investigation scenarios: the purpose of this work is to optimize rendezvous maneuvers in the context of in-space logistics services, such as in-orbit maintenance, propellant depot and refueling, considering the spacecraft trajectory and the dispatching of the limited available resources. The aim is to plant more cost-effective, sustainable and performing missions, with the implementation of the optimized guidance algorithms. The optimized maneuver has been simulated online through Hardware-In-the-Loop (HIL) testing, to investigate the feasibility and robustness of the optimization problem, replicating the 6 degree-of-freedom motion of the spacecraft (in the context of rendezvous maneuvers, the so called “chaser”), through a cooperative robotic arm.