Every year, many workplace accidents occur due to workers’ accumulated fatigue, especially in jobs that require a high level of physical exertion, resulting in stressful environments. This research fits into this contest, with the goal of monitoring physical fatigue: laying the groundwork for developing a device able to determine when a person is too fatigued to continue working safely. To create a controlled environment that induces physical fatigue, each of the 30 volunteers underwent an incremental exercise protocol designed to induce cardio-respiratory fatigue. The protocol was performed on a Garmin bicycle (Tacx Neo Bike Plus) and monitored via a Polar Chest strap (Polar H10).