Femoral fractures pose a significant challenge to public health, especially in light of the increasing number of elderly subjects affected by osteoporosis worldwide. In this context, when aiming to predict femoral fracture in silico, it is still not clear whether muscles actions have a protective effect on the fracture risk or, on the contrary, those increase the fracture risk. Hence, aiming to evaluate the contribution of muscles forces in this context, this thesis focused on exploring various approaches described in the literature to transfer muscles forces from a multibody model to a finite element (FE) model of the femur. In particular, several methods to constrain the FE model were analysed.