Multiple Sclerosis (MS) is a neuro-degenerative disease of the central nervous system, involving motor symptoms such as spasticity, weakness, language disorders and dysphonia; also, fatigue is often considered one of the most debilitating symptoms of MS. An acoustic analysis of MS is performed with vocal material supplied by the speech department of Don Gnocchi hospital (Milan). The data-set includes voice recordings of two subgroups of identical dimension (i.e., sixteen subjects) correspondent to healthy subjects (HS) and MS. Speech material consists in the vocalization of the sustained vowel /a/, the reading of a phonetically balanced speech (Notturno) and one minute of free speech for each subject.