Background: According to the European cancer information system, breast cancer is the most prevalent female cancer in Europe, and it has the highest mortality rate. Thanks to screening programs, the mortality rate has been decreasing in the last decades. The gold standard for breast cancer screening is to perform an X-ray mammography every 2 years for women above the age of 50 years. Therefore, women are periodically exposed to a certain amount of radiation, limiting the screening frequency. This limitation could be overcome by using non-ionizing imaging techniques, increasing the probability of detecting the presence of cancer. A possible solution is given by photoacoustic tomography (PAT), which combines optical absorption contrast with the imaging depth of ultrasound-based techniques.