This thesis focuses on the characterization of the fatigue resistance of bituminous mixtures following the European standards, by means of the four-point bending test. The effects of material type and test temperature on fatigue properties were investigated. The effects of material type were taken into account by considering two different bituminous mixtures, one mixture prepared for a base layer and the other mixture for a binder layer, compacted at a reference air void content. The effect of temperature was investigated by performing tests on the binder course material at two test temperatures, 10 °C and 20 °C. As indicated in the European standard, three strain levels (140 µm/m, 200 µm/m, 300 µm/m) and a minimum of six replicates at each strain level were considered.