Federico Bedini
LAB-ON-CHIP DEVICE FOR HIGH-THROUGHPUT MULTI-ANALYSIS SINGLE-CELL STUDIES.
Rel. Danilo Demarchi. Politecnico di Torino, Corso di laurea magistrale in Ingegneria Elettronica (Electronic Engineering), 2019
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Abstract
Lab-on-chip (LoC) devices embody several key features, including the use of small sample volume, control over fluid dynamics, easy integration of cell manipulation techniques (cell sorting, cell isolation) and higher throughput than conventional analytical methods (e.g. patch clamp) [1-2]. Impedance flow cytometry (IFC) has been widely adopted for high-throughput cell detection and manipulation, sorting and counting. IFC works by measuring the impedance between a set of electrodes as single cells pass through a microchannel [3]. IFC, however, allows only for short-term single-cell analysis. Microelectrode arrays (MEAs) are often used to perform long-term analysis of cell populations. Coupled with electrical impedance spectroscopy (EIS), adhesion, morphology, proliferation and temporal evolution of cells can be analysed [4].
In this work, a novel LoC device is proposed, which integrates in-channel IFC and in-chamber MEAs in the same microfluidic platform
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