In order to obtain a better integration with soft tissue, titanium substrates were functionalized and covered with keratin solution. With the aim of evaluating adhesion properties, tape test and scratch test were performed on titanium surfaces treated with plasma and UV, and covered by solution with different keratin concentrations. The results reveal that keratin coating presents the better adhesion on plasma-treated Ti rather than other surfaces. Then characterization tests were conducted, in order to evaluate surface characteristics. It was evaluated wettability of Ti-cp and plasma-treated Ti with keratin coating using ultrapure water, and wettability of Ti-cp and plasma-treated Ti using keratin solution.