HVO represents an alternative fuel for diesel engines which can help to reduce the pollutants emissions and the CO2 production. In the present thesis work, starting from a conventional 2.3 liter diesel engine for light duty application, a further EGR system was added, namely the Long-Route (Low-Pressure). For each engine point, preliminary tests were performed in order to evaluate the limit values for the main engine parameters, which determined the domain region for the DoEs. The DoEs were prepared by means of MBC Model MATLAB toolbox and then run on the test bench. The acquired data from pollutants analyzers werepost-processed in order to calculate brake specific mass emissions and used to create regression models.