Vasculature analysis is a fundamental aspect in diagnosis, treatment, outcomes evaluation and follow-up in several medical fields. Since vessels branching, maturation or quiescence depend on microenvironment and angiogenic signals, vascular attributes are deeply affected by most of the diseases, from a simple cold to the tumours. Moreover, it was demonstrated that efficient therapies administration leads to attributes normalization. Therefore, vascular network characterization can be a powerful means for earlier pathologies revealing and for their monitoring. For this reason, the development of a non-invasive and quantitative method for the evaluation of blood vessels complexity is a very important issue. Many imaging techniques can be used for visualizing blood vessels, but their application is limited by problems such as high costs, need of contrast agents and use of ionizing radiations.