Microfluidic technology is gaining popularity to build culturing systems. These platforms allow to set up a more physiological relevant environment when compared to static devices, including features such as dynamic flow control and interconnection of multiple tissue cultures. In the context of microfabricated fluidics, a novel system involving an open reconfigurable hanging-drop network for microtissue aggregation and culturing has been proposed by the Hierlemann group at the Bio Engineering laboratory of ETH Zurich. Here, multiple hanging drops of culture media are provided with cells, which sediment by gravity force at the liquid-air interface and form microtissues. Furthermore, drops can be easily connected together with microfluidic channels enabling the analysis of different spheroids interaction.