Sara Di Paolo
Design and fabrication of a microfluidic trap for full spectrum impedance scans of single bacteria.
Rel. Matteo Cocuzza, Maria Dimaki, Winnie Edith Svendsen, Christian Vinther Bertelsen. Politecnico di Torino, Corso di laurea magistrale in Nanotechnologies For Icts (Nanotecnologie Per Le Ict), 2025
|
Preview |
PDF (Tesi_di_laurea)
- Tesi
Licenza: Creative Commons Attribution Non-commercial No Derivatives. Download (50MB) | Preview |
Abstract
The study and characterization of bacteria is nowdays drawing increasing interest among the scientific community. With the use of Impedance Flow Cytometry and Electrical Impedance Spectroscopy is possible to perform detailed analysis on shape, size and compositional properties of bacteria. In the present project, these techniques are intended to be employed in a biosensing device in combination with a customized microfluidic channel, designed with the ambition of trapping single bacteria by exploiting its own geometry. The trap mechanism is first developed and simulated by the means of the finite element method software COMSOL, highlighting the challenges associated with the single bacterium immobilization.
The fabrication of the device components is performed in the cleanroom facility of DTU Nanolab, where, after undergoing several process optimizations, electrodes sets and a silicon mold for the creation of the microfluidic channel are successfully obtained, with the trap width having an average dimension of ∼ 650 nm
Relatori
Anno Accademico
Tipo di pubblicazione
Numero di pagine
Corso di laurea
Classe di laurea
Ente in cotutela
Aziende collaboratrici
URI
![]() |
Modifica (riservato agli operatori) |
