Hydrogels form a class of biomaterials of particular interest to regenerative medicine because of their ability to mimic the extracellular environment in vivo, with the delivery of mechanical support, enhanced permeability to nutrients, and adjustable physicochemical properties. Among the various strategies that have been developed in recent years, human-derived bioactive constituents enable the creation of biocompatible materials free of the immunogenic risk associated with the use of animal-derived additives. In particular, platelet derivatives such as platelet lysate (PL) are a concentrated reservoir of growth factors, cytokines, and bioactive proteins that are commonly recognized to cause cell proliferation, migration, and differentiation. Compared to more traditional platelet-rich plasma (PRP), PL is safer and more standardized because of its cell-free nature and ease of administration in clinical and engineering contexts.