Report sulle startup a impatto in Italia 2025 This thesis explores how Artificial Intelligence (AI) is implemented by innovative startups with significant social and environmental impact (ISSSEIs) in Italy. Grounded in a comprehensive review of literature on social innovation, impact measurement, and responsible AI, the study adopts a qualitative, multiple-case study approach to investigate real-life applications of AI within these mission-driven enterprises. After an initial mapping and assessment phase based on national datasets (SIM, AIDA, Registro Imprese), five ISSSEIs were selected through purposive sampling and interviewed using a semi-structured protocol. The analysis reveals diverse adoption patterns of AI technologies, primarily generative AI and data-driven tools, across internal operations, product development, and strategic planning.