“The World Health Organization” estimates that from 2 to 3 million of lives are saved each year due to vaccination programs (Pollard and Bijker, 2021). This study focuses on Live Virus Vaccines (LVV), which use attenuated live viruses as antigens, to provide a long-term immunity (Robinson, 2016). LVV are not stable in liquid form, so require freeze-drying to preserve the stability and increase their shelf life. Freeze-drying consists of a freezing step in which the aqueous solution is brought to freeze before water is removed by an initial sublimation step (primary drying), followed by a desorption step of bound water (secondary drying).