A Peristaltic Pump is a Positive Displacement pump, named after peristalsis, which is the progressive constriction and relaxation of a tube. These pumps play a crucial role in supporting the development of more reliable in vitro models, by replicating physiological dynamic conditions and shear stresses. This master thesis, conducted in collaboration with the Company IVTech srl, focused on designing and developing a peristaltic pump head prototype, to replace the commercial head model currently in use in the IVTech Live Flow system. The pump head prototype was designed using a 3D modelling software, and each component was developed according to company specifications.