In order to optimize therapies aimed at tissue regeneration, it is essential to have access to e predictive and reliable in vitro models. Such models enable effective preclinical testing in accordance with the 3Rs principle, overcoming the limitation of 2D cell cultures and animal testing. 3D cell culture is a promising alternative to the current state-of-the-art, enabling the creation of more reliable 3D in vitro tissue models. Nowadays, advancements in microfluidics have led to platforms known as organ-on-a-chip, aimed at the in vitro study of engineered tissue models. According to the scaffold-based model of tissue engineering, these constructs have the aim to create a temporary structure that physically supports cells during tissue formation, providing biological and chemical cues.