The use of binaural beats (BB) for neurostimulation is an innovative technique useful for regulating brain activity and relieving stress. The aim of this thesis is to develop a closed-loop neurostimulation protocol to investigate whether the use of BB adapted to the subject’s stress in real-time can improve cognitive performance and modulate stress in healthy individuals. This study evaluate whether the adapted stimulation with BB in the alpha band is more effective compared to stimulation with constant BB and to the control condition without stimulation. The research included the validation of artificial intelligence models to identify stress levels in real time using physiological signals such as electroencephalogram (EEG) and the electrocardiogram (ECG).