Climate change is affecting the water availability and the temperature of rivers, leading to modifications in their biodiversity and posing new challenges for water management. This thesis focuses on investigating the habitat availability for temperature-sensitive fishes during a low-flow period, as part of the T°Cino project aimed at identifying mitigation strategies for the arising problems in the Ticino River (Italy). This study was performed in two sections of the river (Turbigo and Vigevano), located in northern Italy, which is an area significantly impacted by flow regulation and climate change. Through field surveys and the implementation of the MesoHABSIM methodology, the habitat suitability for five different species (bullhead, Italian spring goby, Italian vairone, Po brook lamprey, and marble trout) was assessed to contribute to the T°Cino project’s objectives.