Malignant tumors are some of the most serious diseases affecting human health, which clinical prognosis remains relatively poor. Therefore, there have been intensive efforts in the past years directed at the discovery and the development of new small molecules, which are in most cases natural compounds, capable of interfering with tubulin polymerization and therefore leading to their anticancer potential. In facts, microtubules are involved in various cellular processes such as cell division, cell motility and intracellular transport. Their essential role in cell division makes them a fundamental target for designing anti-mitotic agents. Microtubules are composed of αβ tubulin subunits that consist of various isotypes encoded by specific genes which are expressed in a tissue-specific manner.