Muscle synergies represent a model about how the Central Nervous System (CNS) recruit different muscles, coordinating their activation during a functional task. Muscle strategies are usually estimated from surface EMG signals, recorded with bipolar technique, from the group of muscles involved in the movement under study; usually one bipolar detection system per muscle is used. However, studies in literature have suggested that some lower limb muscle (Rectus Femoris, Gastrocnemius Medialis, Vastus Medialis, Soleus) are not uniformly activated during a motor task, but separated regions are selectively recruited in different phases of the movement. This study aims to evaluate if muscle synergies extracted during gait and cycling depend on the electrode positioning on the muscle.