Over the course of the last decades, nanoparticles have been objects of many researches because of their remarkable properties derived by the dimensions within the nanoscale. The study of their interactions is crucial for their understanding and their implementation in many significant applications such as drug delivery systems, biosensing, clinical and biomedical diagnostics. Cryogenic Electron Tomography (Cryo-ET) is used to evaluate the long-range colloidal interactions of gold nanoparticles within the DLVO (Boris Derjaguin, Lev Landau, Evert Verwey and Theodoor Overbeek) theoretical framework. Cryo-ET is based on the 3D reconstruction of a vitrified nanoparticle dispersion, allowing to derive the 3D radial distribution function of the nanoparticles from which the mean interaction potential and the structure factor can be calculated.