Butanol and isopropanol are currently produced by the petrochemical route. The production of bio-butanol and bio-isopropanol can also be carried out by fermentation (IBEA production: Isopropanol – Butanol – Ethanol – Acetone). However, the alcohol concentration in the fermentation broth obtained is less than 24 g/L, mainly due to the inhibition of butanol. The most used system to purify the IBE leaving the fermenter is distillation but, given the very low concentrations of the distillation feed, the separation costs can be very high. The main objective of this work is the optimization of the process to minimize the separation cost. The optimization process begins with the optimization of the number of plates, the feeding plate, the feeding temperature, and the heat exchanger technology.