The impact of the entrepreneurial ecosystem on the fit of the Italian start-ups into the value chain. The analysis of a generic entrepreneurial ecosystem is a though challenge, since it includes different variables and players extremely correlated among them. The aim of this study is to focus strictly on the relationships happening between the start-ups and the other actors in the ecosystem. Since many researchers – mentioned in the literature review – already studied broadly and deeply these dynamics separately, this analysis wants to bring new concrete conclusions with a new perspective. In particular, it will look to how these relationships affect what at the end is the crucial parameter of a start-up survival: its fit into the value chain.