In recent years, restorative dentistry has increasingly focused on understanding the interactions between restorative materials and dental tissues, since the long-term stability and clinical success of dental restorations depend largely on the integrity of the adhesive interface. In this context, dentin represents a complex biological substrate whose chemical composition and structural organization can be significantly altered by endodontic irrigation protocols. Specifically, commonly employed solutions such as sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA), while essential for the cleaning and disinfection of the dental root canal, can induce alterations in both the organic (collagen) and inorganic (apatite) components of dentin. These alterations negatively affect the formation and stability of the hybrid layer and, consequently, the overall durability of the dental restorations.