In the context of D3 4 HEALTH Foundation research on digital health technologies, this work contributes to the development of innovative tools for biomedical applications, with potential impact on diagnosis, monitoring and treatment. The aim of this thesis is to optimize a multi-layer microfluidic platform for cell culture and analysis, with the integration of Organic Electrochemical Transistors (OECTs) for real-time monitoring of cellular responses. Starting from a previously developed microfluidic device, the design was modified to enable the culture of a human skin cell model and the incorporation of OECT sensors. The methodology involved the CAD design of the microfluidic platform composed of three different layers: two for cell culture and microfluidic connections, with a central chamber aimed at hosting the barrier model on a porous membrane, and the third one for bottom sealing of the chamber and OECTs integration.