The conducted study investigates advanced methods for non-invasive physiological data acquisition and their application in stress assessment. The research explores the potential of the Nanomade Pulse sensor, a novel high-sensitivity strain gauge technology, for monitoring cardiac (Inter-Beat Interval, IBI or Heart Rate, HR), respiratory (Inter-Respiratory Interval, IRI or Respiratory Rate, RR), and movement (Actimetry, ACT) parameters. The initial phase involved validating the Nanomade Pulse sensor demo-kit against reference devices (Polysomnography, Garmin Venu 2 Plus, and Polar H10) in a controlled study with 11 participants (9 females and 2 males), aged 24 ± 3 years, under different postural and metabolic conditions. Although the sensor demonstrated promising capabilities, the data quality proved not even consistent for accurate Heart Rate Variability (HRV) analysis, highlighting the current technological limitations of the sensor demo-kit.