Skeletal muscle (SkM) is a highly organized tissue displaying multiple levels of structural organization, whose development and contractile function is intimately related to its anisotropic structure. To engineer SkM in vitro models, scaffolds must mimic the native extracellular matrix (ECM) architecture, providing not only bio-chemical but also physical cues to promote myoblasts alignment and maturation. Hydrogels provide a water-rich, biomimetic 3D environment, and represent the most promising candidate materials to engineer scaffold for tissue engineering applications. However, the intrinsic isotropy inherent to their network is not capable of guiding cell alignment, limiting their applicability to those tissues characterized by high level of structural anisotropy, like skeletal muscle.