The excessive usage of antibiotics is driving the rise of antibiotics resistance. The drug susceptibility testing (DST) based on culture for drug resistance detection is the gold standard for managing the Mycobacterium tuberculosis infection. However, these tests are manual and require time, sophisticated laboratory infrastructure and qualified staff able to use the instrument. To provide an alternative to manual DST, in recent years, many software have been created, including Mykrobe, an open source software, that performs anti microbial resistance predictions (AMR) on microbial deep sequencing data. This prediction is performed in a few minutes and doesn’t require qualified staff to run it.