Mattia Tarchini Bojczuk
A Minimal Model of Inoculum-Dependent Growth in Cancer Cell Cultures.
Rel. Carla Bosia. Politecnico di Torino, Corso di laurea magistrale in Physics Of Complex Systems (Fisica Dei Sistemi Complessi), 2022
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Abstract
In recent years the tools of statistical physics have been successfully employed in dealing with the stochastic phenomena that have surfaced in the context of microbial population studies. In a given environment a cell has access to a large set of physiological macrostates (or phenotypes) in a similar way to how a degrees-of-freedom-rich physical system in contact with a heat reservoir can be found in one of its macroscopic configurations. The statistical laws that govern how such state spaces are explored are believed to share similar principles. In particular, how fluctuations in the environment, possibly dependent on population size, might be related to the phenotypic variability (represented by maximum growth rate variability) observed in population ensembles appears to be a relevant problem suited to a statistical physics approach.
In a study co-authored by the relators of this thesis, the extent of such dependence was tested by looking at how population parameters of two widely used cancer cell lines ( Jurkat and K562), grown in fixed carbon-limited media, were modulated by the size of the initial density
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