Francesco Chierici
Data analysis and model development for a photonic interrogation platform for cancer chemotherapy optimization using power spectrum analysis of sub-cellular dynamics.
Rel. Jacek Adam Tuszynski, Aristide Dogariu. Politecnico di Torino, Corso di laurea magistrale in Ingegneria Biomedica, 2021
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Abstract
The aim of this project is to propose a possible new platform for cancer therapy optimization based on Dynamic Light Scattering (DLS), a non-invasive, low-intensity photonic interrogation technique. Firstly, the system is used to investigate properties of different cell lines without administering drugs to see differences in cell lines and cell states as a proof of their sensitivity with respect to cellular dynamics. Afterwards, DLS is used to investigate cancer cells under an incrementally-increased dose delivery of a therapeutic agent in order to precisely determine its apoptosis-inducing concentration. The platform takes advantage of nano-scale technology and DLS in order to measure the cell dynamics in real time as function of drug dose, ranging from pM to mM, and to obtain the dose-response profile.
This allows to better understand the drug efficacy, its side effects and the subcellular dynamics, possibly helping the development of more patient specific therapies because both dose determination and drug administration scheduling can be optimized with this methodology
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