Alternative Splicing is the RNA's ability to be spliced into many different mRNA isoforms, and it is of great evolutionary importance because it allows a single gene to produce a variety of proteins. However, in cancer, the spliceosome machinery produces aberrant isoforms or changes their expression, which alters the behavior of the cell, as they interfere with biological pathways. The study of novel cancer isoforms is essential for developing therapies that can suppress their expression or exploit the new epitopes, in addition to providing a deeper understanding of the disease. The relatively new long-read sequencing technology enables a more accurate representation of the transcriptome than the older short-read.