The intensification of climate change has stimulated the research for sustainable energy sources, among which second generation (2G) bioethanol has emerged as a promising alternative. This biofuel can be obtained through the fermentation of simple sugars derived from lignocellulosic biomass. A critical step in the biomass hydrolysis process is the liquefaction, where pretreated biomass is converted into glucose through the action of enzymes. To ensure economic feasibility, this step must be conducted at high solids loading to minimize water usage and reduce the energy demand for distillation of ethanol in the last step of the process. However, under such conditions the biomass slurry exhibits non-Newtonian behaviour, leading to significant challenges in the mixing process and in the description of system evolution during enzymatic hydrolysis.