Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest tumours that affect the exocrine pancreas and it has a poor survival rate. These unfortunate data are ascribed to the absence of symptoms in the early stages, invasive metastasis, and high resistance to the therapeutic options. For these reasons, in vitro models that can reproduce the microenvironment of the pancreatic tissue are required to develop therapies and to predict drug safety and efficacy in humans. Organ-on-chips can be a powerful technology to achieve these goals. In this thesis project, a microfluidic device for modelling the pancreatic acino-ductal unit, is developed. The function of the platform is to investigate the crosstalk between PDAC and the stromal components which plays a fundamental role in cancer progression.