One-photon (1P) imaging and optical stimulation are two powerful tools widely used to assess and monitor the behaviour of individual and multiple neurons in in-vivo and ex-vivo experiments. However, standard 1P microscopes are limited by diffraction and scattering, which reduce the maximum optical depth achievable to few hundreds of micrometres deep into brain tissues. To overcome this issue, implantable waveguides and probes (i.e. optical fibres) are commonly used, but they are highly-invasive and may lead to damage the surrounding tissue. In this work, it has been developed a customized 1P setup fully compatible with an innovative acousto-optic technology based on an ultrasonic phased-array, which allows to sculpt a non-invasive virtual waveguide within the tissue, by locally changing its refractive index.