Articular cartilage (AC) is an avascularised and highly specialized tissue, populated by only one cell type, the chondrocytes. Behind its simple and homogenous appearance, this tissue hides a heterogeneous composition with a high level of organisation and biomechanical properties that makes it difficult to be repaired when there are failures. Hydrogels, 3D polymeric networks, are identified as ideal structures for their ability to recapitulate the cartilage solid/liquid ratio and to provide an appropriate niche, scaffolding and environmental bioactive signals for cells. Several natural polymers have been analysed for hydrogel manufacturing. Among them, chitosan, a biocompatible and biodegradable polysaccharide, has shown promising features for reproducing cartilage Extracellular Matrix (ECM) for its structural similarity to cartilage glycosaminoglycans (GAGs) and its mechanical properties, inducing mesenchymal stem cell (MSCs) chondrogenic differentiation.