Sustainability has been the new global purpose from the last decades due to environmental concerns about the use of non-recoverable natural resources. The interest in using bio-materials in constructions and, in particular, in pavement engineering has grown significantly, heading us to concentrate on finding recoverable materials with the highest performances. Roads are mainly composed of asphalt mixture, a combination of aggregates and bitumen, generally petroleum-based. Nowadays the tendency is to abandon these petrochemical products in a favour of environmental friendly materials, called BioBinders. In this thesis, two different binders were studied and compared: a non-modified, neat and petroleum-based binder, defined as a target, and a bio-materials recently introduced in the market: BioPhalt®.