This work presents a microfluidic chip used to perform multiplexed analysis in order to diagnose infectious diseases at an early stage. This microfluidic approach uses two layers to attain a nanolitre scale compartmentalisation of the sample in four chambers requiring no pumps or valves. The device was designed using a 3D CAD software and fabricated by a stereolithography (SLA) 3D printer in a high temperature and transparent Polycarbonate-like material (Accura SL 5530). This design was experimentally validated by loading the sample solution within the fluidic channels under mineral oil. Surface wettability was ultimately investigated performing a contact angle evaluation according to the sessile-drop method.