Physiological in vitro kidney models have significant potential in advancing the field of renal physiology and drug-induced nephrotoxicity studies by reducing the reliance on animal testing. This master’s thesis project aims to develop a 3D in vitro model of the kidney proximal tubule using 3D printing and bioprinting techniques. Current in vitro models of the proximal tubule are limited in their ability to replicate native microarchitecture and cellular functionality. This project focuses on the development of a perfusable in vitro model capable of mimicking the structural features of the tubular interface while providing a suitable microenvironment for cell culture. Gelatin methacryloyl (GelMA) was synthesized and prepared at different concentrations, combined with hyaluronic acid derivatives to enhance the physicochemical and mechanical properties of the hydrogel scaffolds.