Today, one of the main goals of medical applications is to improve therapies’ effectiveness while reducing the costs and patients’ pain. The golden standard for medical diagnosis is based on blood sampling, given its cost-effectiveness and efficiency. However, some diseases such as diabetes require continuous monitoring of particular blood parameters, making blood sampling difficult and extremely painful. Moreover, the continuous glucose monitoring (CGM) devices available on the market are quite invasive since they rely on hypodermic needles about 5mm long. This work focuses on the development and characterization of a glucose biosensor based on microneedles (uNs) offering a promising alternative to the state-of-the-art CGM devices, thanks to its minimal invasiveness.