The evaluation of drug efficacy and safety still relies predominantly on two-dimensional (2D) cell cultures and animal models, both of which present significant limitations. Conventional 2D cultures fail to reproduce the complex three-dimensional (3D) architecture and mechanical cues of native tissues, while animal testing remains expensive, ethically debated, and often poorly predictive of human responses. Within this framework, the implementation of bioreactors, systems designed to regulate and monitor the cellular microenvironment, is increasingly crucial to replicate physiological conditions and produce 3D reliable in vitro tissue models for preclinical drug evaluation. Indeed, physical forces within microenvironments are key determinants of stem cell behavior and play a decisive role in tissue growth and maturation.