Myocardial bridging (MB) is a coronary artery anomaly characterized by an intramyocardial course of a coronary segment, resulting in dynamic luminal compression during systole. Although traditionally considered benign, increasing evidence has associated MB with altered coronary hemodynamics and associated increased risk of atherosclerotic plaque formation, potentially culminating in myocardial ischemia or infarction. This study aimed to establish a personalized framework for the morphological characterization of MB segments by (i) assessing the impact of operator-dependent variability on the reconstruction of patient-specific MB geometries, and (ii) conducting a detailed morphological quantification of the reconstructed arterial models. To do that, the left anterior descending coronary artery of three ostensibly healthy individuals was imaged using dynamic computed tomography (CT) across multiple phases of the cardiac cycle, sampled at 10% intervals (P1: 40–80%; P2 and P3: 10–100%).