In recent years, extracellular vesicles (EVs) produced by organisms from all three domains of life have increasingly attracted the interest of the scientific community. Their favourable characteristics - small size, ability to circulate in biological fluids, and the presence of a variety of bioactive molecules within their cargo - make them promising candidates for drug delivery and immune intervention strategies. This work arises from the need to explore the potential of yeast EVs as novel nanocarriers for drug delivery. In particular, EVs produced by Saccharomyces cerevisiae were studied. S. cerevisiae is a well-established model organism that shares several features with more complex eukaryotes, making it particularly suitable for preliminary studies on EVs.