Despite about one-third of bone metastases affect vertebrae, compromising bone integrity, the most used clinical score to estimate the risk of instability or fracture of a metastatic spine, i.e. the Spinal Instability Neoplastic Score (SINS), does not consider vertebral biomechanics. This thesis is a part of a project of the Bioengineering and Computing Laboratory of Rizzoli Orthopedic Institute that aims to complement SINS by validated subject-specific finite elements models (SSFEMs) built from routinely collected CT images. The main object of this thesis was the computational part of the validation of SSFEM vertebral strains and displacements against experimental measurements. T8 vertebrae from two donors were tested in intact and lesion conditions; these latter were simulated with dome-shaped, endplate-centred holes of increasing size (d = 6, 10, 15, 20 mm) to represent a progressively worsening lytic lesion.