This thesis presents a comprehensive theoretical and experimental study of ultra-fast laser-assisted micromachining of borosilicate glasses: Schott BOROFLOAT® 33 and EAGLE XG®. BOROFLOAT® 33 is a clear and transparent colorless glass. Its remarkable transmission and its minimal fluorescence intensities over the light spectrum make BOROFLOAT® 33 ideal for different applications in high-performance photonics and microscopy. Since it is highly resistant to attack by chemicals, alkalis, and organic substances, it is powerful for lab-on-a-chip devices where precise control over microfluidic channels is essential. Lastly, its high thermal shock resistance and low thermal expansion yield applications that require good temperature stability. EAGLE XG® glass is a borosilicate glass specifically designed for high-performance LCDs.