Within the huge family of sensors and biosensors technology, electrochemical sensors allow the transduction of a chemical information to an electrical signal to precisely characterize complex chemical phenomena, such as redox reactions, or to quantify the concentration of a molecule in a solution. Nanostructured metal oxides, with spinel crystalline structure, are attractive for electroanalytical methodologies because they could improve the catalytic activity of the redox process of some molecules: they could have the ability to enhance the detection' sensibility. Therefore, the aim of this work is to study the electrochemical performance of nanostructured metal oxides (i.e. zinc chromite, zinc ferrite and copper ferrite) in the detection of Cyclophosphamide, a very well-known anticancer drug, whose metabolism relies on Cytochrome P450.