This research aimed to investigate the effects of two distinct light exposure systems, the Bioptron device (hyperpolarized light) and the Vielight NeuroPro device (InfraRed radiation), on cellular responses in three cancer cell lines: PC3, HeLa, and MCF7. The study focused on elucidating the impact of different irradiation conditions on cell viability, morphological changes, ATP production, and metabolic shifts. Cells were cultured in standard conditions and exposed to light using the Bioptron device (40 mW/cm2) or the Vielight NeuroPro device (60 mW/cm2, 10 Hz frequency sweep). Exposure times ranged from 10 to 60 minutes, with some groups receiving intermittent breaks. Cell viability was assessed using the Alamar blue assay, while morphological changes were evaluated through immunofluorescence staining of actin, tubulin, and mitochondria.