Nowadays, the fast development of manufactural technologies and high demand of new drugs and therapeutics, make more and more essential a step forward for cell culture systems and organ and tissue in vitro models. Indeed, current preclinical models such as animals or 2D cell cultures are poor predictors of the tested drug’s effect in humans. The differences in the great part of human physiological tissues structures compared to animals and the over-simplified model lacking all the main features of in vivo microenvironment in the case of 2D models, have brought the scientific community to ask for more representative and precise models in order to obtain more reliable results while reducing laboratory animal testing according with the 3R principles.