Natural complexity arises from the organization of individual elements into larger systems. This hierarchy, found at different size scales, contributes to the overall properties and functionalities of these systems. Another important concept in nature is anisotropy, which refers to the directional dependence of physical properties. Anisotropic structures are commonly found in tissues of the human body, providing specific physical properties in preferred orientations. This study aimed to develop a new bioink for 3D bioprinting that replicates the alignment of native anisotropic tissues. A composite bioink using alginate and modified gelatin was optimized as the hydrogel matrix, along with various micro/nanofillers.The reinforced bioink design consists of fabricating and characterizing the micro/nanofibers, optimizing the hydrogel component, and incorporating the micro/nanofillers into the hydrogel.