Skin models are useful in vitro tools to study the development of skin pathologies and to screen and develop new specific drugs. They are becoming an alternative to animal models which are often ineffective in reproducing features of human skin due to difference in the evolution. Additionally, the animal testing ban rise the necessity to develop models that closely mimic in vivo-like environment in vitro. Skin has a very complex structure and physiology, therefore, challenges associated to composition, architecture and cellular organization are to be addressed if aiming at replicating its native properties. Among the organisational requirements of a vascularised in vitro skin model, endothelial cells must be incorporated in the dermal compartment together with fibroblasts.